Fedbatchdesigner: A User-Friendly Dashboard for Modeling and Optimizing Growth-Arrested Fed-Batch Processes

Author(s)
Andrea C Graf, Julian Libiseller-Egger, Mathias Gotsmy, Jürgen Zanghellini
Abstract

Optimizing fed-batch fermentation strategies is key to maximizing bioprocess efficiency. While mathematical modeling can aid process design, its complexity often limits accessibility for experimental scientists. We present FedBatchDesigner, a user-friendly web tool for optimizing fed-batch processes with a growth-arrested production stage. With minimal input requirements, FedBatchDesigner enables rapid exploration of a process's titer, rate, and yield (TRY) landscape for constant, linear, and exponential feeding strategies. Interactive visualizations allow users to assess trade-offs between productivity and titer, supporting rational decision-making without the need for extensive modeling expertise. We demonstrate FedBatchDesigner's utility via two case studies: synthesis of (i) l-valine with a microaerobic production stage in Escherichia coli and (ii) ethanol under nitrogen starvation in Saccharomyces cerevisiae. FedBatchDesigner is freely available at chemnettools.anc.univie.ac.at/FedBatchDesigner, with the source code provided at github.com/julibeg/FedBatchDesigner under the MIT license.

Organisation(s)
Department of Analytical Chemistry
External organisation(s)
acib – Austrian Centre of Industrial Biotechnology, Vienna Doctoral School in Chemistry (DoSChem), Eidgenössische Technische Hochschule Zürich
Journal
ACS Synthetic Biology
ISSN
2161-5063
DOI
https://doi.org/10.1021/acssynbio.5c00357
Publication date
07-2025
Peer reviewed
Yes
Austrian Fields of Science 2012
104027 Computational chemistry, 106005 Bioinformatics
Portal url
https://ucrisportal.univie.ac.at/en/publications/51ca3d05-5c10-44f9-baaf-63c88e928ef2