Optimizing VLP production in gene therapy: Opportunities and challenges for in silico modeling
- Author(s)
- Leopold Zehetner, Diana Széliová, Barbara Kraus, Michael Graninger, Jürgen Zanghellini, Juan A. Hernandez Bort
- Abstract
Over the past decades, virus-like particle (VLP)-based gene therapy (GT) evolved as a promising approach to cure inherited diseases or cancer. Tremendous costs due to inefficient production processes remain one of the key challenges despite considerable efforts to improve titers. This review aims to link genome-scale metabolic models (GSMMs) to cell lines used for VLP synthesis for the first time. We summarize recent advances and challenges of GSMMs for Chinese hamster ovary (CHO) cells and provide an overview of potential cell lines used in GT. Although GSMMs in CHO cells led to significant improvements in growth rates and recombinant protein (RP)-production, no GSMM has been established for VLP production so far. To facilitate the generation of GSMM for these cell lines we further provide an overview of existing omics data and the highest production titers so far reported.
- Organisation(s)
- Department of Analytical Chemistry
- External organisation(s)
- Baxalta Innovations GmbH
- Journal
- Biotechnology Journal
- Volume
- 18
- ISSN
- 1860-6768
- DOI
- https://doi.org/10.1002/biot.202200636
- Publication date
- 07-2023
- Peer reviewed
- Yes
- Austrian Fields of Science 2012
- 304005 Medical biotechnology, 106005 Bioinformatics
- Keywords
- ASJC Scopus subject areas
- Applied Microbiology and Biotechnology, Molecular Medicine
- Sustainable Development Goals
- SDG 3 - Good Health and Well-being
- Portal url
- https://ucrisportal.univie.ac.at/en/publications/81a552aa-60ac-4eff-b4bf-62893693fbec